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**Research has shown that certain compounds in cannabis, such as cannabinoids CBD and THCV, can activate transient receptor potential (TRP) channels, specifically TRPA1 ion channels, which are associated with coughing

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These changes suggest that flue-cured tobacco enhances its stress adaptation through various metabolic regulatory strategies under chilling stress

Indeed, while primary cultures may better mimic a physiological behavior, they are associated with technical challenges (lack of tissue availability, specific handling required and donor-specific variations) [19, 40]