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Some studies (Han et al., in vitro study has also suggested that folate had a dose-dependent protective effect on neurons (Chen et al., Previous epidemiological research mainly explored the relationship between homocysteine, folate, and vitamin B12 and neurodevelopment in children with ASD using correlation analyses (Guo et al., The major strength of our study was that multiple variable regression models with confounder control were used to assess the associations between serum homocysteine, folate, and vitamin B12 levels and the outcomes and severity of ASD
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