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The increase in fecal butyrate levels observed likely reflects the increase in gross energy of feces observed as ambient temperature drops 6

Reduced postprandial glucagon secretion

The BIOM file output from the DADA2 pipeline and the DECIPHER-aligned FastTree phylogenetic tree were input into QIIME (v1.9.1) and the scripts in the Supplementary Materials (QIIME Scripts compilation) were used to establish the alpha diversity metrics of Chao1 richness estimate, Simpsons evenness measure E, Shannon diversity index H, Simpsons diversity index, and Faiths phylogenetic diversity metric (whole tree) as well as the beta diversity measures of unweighted and weighted UniFrac analyses (the latter of which factors in ASV abundance 37 ) and BrayCurtis dissimilarity analysis

[DOI] [PMC free article] [PubMed] [Google Scholar] 101.Chesterman T., Thynne T.R