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siRNA Transfection in vitro When HBE cells reached 3050% confluence, they were transfected with SREBP2-siRNA (sc-36559, SantaCruzBiotechnology, USA), HMGCR-siRNA (sc-44851, SantaCruzBiotechnology, USA), ABCA1-siRNA (sc-41137, SantaCruzBiotechnology, USA) or Control-siRNA (sc-37007, SantaCruzBiotechnology, USA) at 10 nM

To determine the required sample size, the formula n = 100 + 50i was used, where i is the number of independent variables in the final model [82]

Sublingual Glands: On either side of the tongue, just below the floor of the mouth, are the sublingual glands

It would seem that the earliest cigar box instruments would be crude and primitive, but this is not always the case