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Despite early career success, her health gradually deteriorated from the combined effects of smoking, poor nutrition, and her generally excessive lifestyle

coli To determine if immune cells in the blood from smokers were more or less responsive to an ex vivo challenge, we incubated freshly isolated whole blood samples for 7 h (to obtain levels of both early and late cytokines 21 ) in a 5% oxygen (to mimic in vivo conditions), humidified incubator at 37 C in the presence or absence of an immune cell challenge

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