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Left lungs were homogenized using the Qiagen Tissue Lyser II, followed by simultaneous DNA and RNA isolation using Invitrogen DNA and RNA isolation kits
The tobacco industry is cited as the biggest barrier to reducing tobacco use
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thats how much you bother me. In 1980-1981, Ladd was in 23 episodes of the spin-off TV series called Alice, not as Flo but as Isabelle Belle Dupree, a sharp-tongued but kind-hearted waitress, given the catchphrase, Butter my biscuits! Although she won a Golden Globe for her performance, the character was written out after two seasons, reputedly because she clashed with her co-stars
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