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In one such study, researchers screened seven oral nicotine-delivery products, including nicotine replacement therapy (NRT) products and PMIs nicotine pouches, commercialized as ZYN , for 43 HPHCs potentially present in tobacco products
After deparaffinization, antigen retrieval and permeabilization, the samples were blocked and probed with the rabbit polyclonal antibodies against loricrin, involucrin (both Abcam, UK), filaggrin, KLK7 and TG1 (all three Invitrogen, USA) and appropriate anti-rabbit secondary antibody conjugated with Alexa Fluor 555 (Invitrogen, USA)
no such effect was seen on heart rate and arterial stiffness in the volunteers who had smoked e-cigarettes without nicotine
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