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Washes were performed by diluting enzyme 1:20 in buffer and concentrating in a 50 kDa Amicon Ultra 0.5 centrifugal filter device by centrifugation for 10 mins at 14,000 x g , performed at 4 C

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Cancer Risk Starts Rising Sharply Smoking for 5 years increases: Lung cancer risk by 3040% Throat cancer risk significantly Risk of chronic inflammation and pre-cancerous lesions Breathing Capacity Drops Many smokers experience: Shortness of breath on exertion Wheezing Reduced stamina persistent phlegm HRCT scans often demonstrate early emphysema, airway obstruction, and bronchiolar thickening

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